ISSN 1673-8217 CN 41-1388/TE
Supervisor:China Petrochemical Corporation Limited Sponsor:Sinopec Henan Oilfield Company
Volume 34 Issue 05
Dec.  2020
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YANG Fan, XU Kewei, GU Lei, NING Lirong, LU Li, SHEN Zhongmin. 2020: Simulated experimental research on quantity change characteristics of oil and gas microorganisms. Petroleum Geology and Engineering, 34(05): 44-48.
Citation: YANG Fan, XU Kewei, GU Lei, NING Lirong, LU Li, SHEN Zhongmin. 2020: Simulated experimental research on quantity change characteristics of oil and gas microorganisms. Petroleum Geology and Engineering, 34(05): 44-48.

Simulated experimental research on quantity change characteristics of oil and gas microorganisms

  • Received Date: 2019-10-09
  • Rev Recd Date: 2020-04-27
  • Publish Date: 2020-12-08
  • Based on the surface soil samples of an oil and gas area in Jiangsu oilfield, a gas reservoir and reservoir simulation environment with certain oil and gas background information was constructed. The quantity change characteristics of oil and gas microorganisms under different light hydrocarbon seepage conditions were studied through simulation experiments by using surface soil samples from a certain area in Jiangsu oilfield. The results showed that the number of microorganisms using hydrocarbons as carbon source increased rapidly and finally reached a plateau stage, while the number of microorganisms without hydrocarbon supply decreased gradually. The number of microorganisms was proportional to light hydrocarbon leakage flux in a short time, however, after long-term domestication under different leakage flux, the number of microorganisms tended to be uniform. The abundance of gas indicator gene pmoA and oil indicator gene bmoX have good response and specificity to gas and oil reservoir environment respectively. Microorganisms were in a state of extinction under the simulation condition of exhausted reservoir without hydrocarbon supply. Through simulation experiments, the response of surface oil and gas microorganisms to underlying reservoirs is verified, and the quantitative variation characteristics of oil and gas microorganisms under light hydrocarbon seepage environment are preliminarily clarified.
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  • EHRLICH H L.Geomicrobiology[M].New York:Marcel Dekker,2002.
    丁力,吴宇兵,刘芬芬.中拐凸起火山岩油气藏微生物地球化学勘探研究[J].特种油气藏, 2018, 25(4):24-28.
    梅海,林壬子,梅博文,等.油气微生物检测技术:理论、实践和应用前景[J]. 天然气地球科学, 2008, 19(6):888-892.
    ENGEBRETSON J J,MOYER C L.Fidelity of select restriction endonucleases in determining microbial diversity by terminal-restriction fragment length polymorphism[J].Appl Environ Microbiol,2003,69(8):4823-4829.
    杨帆,沈忠民,梅泽.甲烷氧化菌特异性培养基研究[J]. 应用与环境生物学报, 2016, 22(6):1127-1133.
    KOHNO T,SUGIMOTO Y,SEI K,et al.Design of PCR primers and gene probes for general detection of alkane-degrading bacteria[J]. Microbes and Environments, 2002, 17:114-121.
    SHENNAN J L.Utilisation of C2-C4 gaseous hydrocarbons and isoprene by microorganisms[J].J Chem Technol Biotechnol,2006,81:237-256.
    KLOOS K,MUNCH J C,SCHLOTER M.A new method for the detection of alkane-monooxygenase homologous genes (alkB) in soils based on PCR-hybridization[J].Journal of Microbiological Methods,2006,66:486-496.
    刘宇坤,何生,何治亮,等.碳酸盐岩超压岩石物理模拟实验及超压预测理论模型[J].石油与天然气地质, 2019,40(4):716-724.
    HUMER M,LECHNER P.Alternative approach to the elimination of greenhouse gases from old landfills[J].Waste Management and Research,1999,17:443-452.
    MEGRAW S R,KNOWLES R.Methane production and consumption in a cultivated humisol[J].Biology and Fertility of Soils,1987,5:56-60.
    BOECKX P,CLEEMPUT O V.Methane oxidation in a neutral landfill cover soil:influence of moisture content, temperature and nitrogen-turnover[J].Journal of Environmental Quality,1996,25:178-183.
    KOLB S,KNIEF C,STUBNER S,et al.Quantitative detection of methanotrophs in soil by novel pmoA targeted real-time PCR assays[J]. Appl Environ Microbiol, 2003, 69:2423-2429.
    YAN T, YE Q, ZHOU J, et al. Diversity of functional genes for methanotrophs in sediments associated with gas hydrates and hydrocarbon seeps in the gulf of Mexico[J].FEMS Microbiol Ecol,2006,57:251-259.
    ZHANG F,SHE Y,ZHENG Y,et al.Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China[J].Appl Microbiol Biotechnol,2010,86:1183-1194.
    ROJO F.Minireview:degradation of alkanes by bacteria[J] Environmental Microbiology,2009,11:2477-2490.
  • [1] LIN Tao, SUN Yongtao, SUN Yubao, SONG Hongzhi, LIU Haitao, LI Tianliang, 2020: Optimization of moderate gas injection in heavy oil thermal recovery——by taking A oil reservoir in Bohai oilfield as an example, Petroleum Geology and Engineering, 34, 46-49.
    [2] SUN Zhigang, YANG Haibo, ZHANG Hongxin, WANG Jian, WANG Rui, ZHANG Bo, 2019: Physical simulation experiment of injection-production coupling, Petroleum Geology and Engineering, 33, 68-70.
    [3] , , , , , , 2017: , Petroleum Geology and Engineering, 31, 41-43+46.
    [4] , 2017: , Petroleum Geology and Engineering, 31, 105-107.
    [5] Wang Peiwen, , , , , 2016: Laboratory study on influencing factors of CO_2 huff and puff in heavy oil reservoir, Petroleum Geology and Engineering, 30, 115-118+150.
    [6] , , , , , 2015: , Petroleum Geology and Engineering, 29, 127-130.
    [7] , , , , , 2013: , Petroleum Geology and Engineering, 27, 118-120.
    [8] , , , , , , 2011: Field test on microbial flooding of ultra-low permeability reservoir in Ansai oilfield, Petroleum Geology and Engineering, 25, 115-117+121+146.
    [9] , , , 2011: , Petroleum Geology and Engineering, 25, 118-120.
    [10] , , , 2011: Study on microbial prospecting of oil and gas of Honghe 26-37 well block in Zhenjing oilfled, Petroleum Geology and Engineering, 25, 29-31+6.
    [11] , , 2010: , Petroleum Geology and Engineering, 24, 29-32.
    [12] , , , 2010: , Petroleum Geology and Engineering, 24, 124-126+129.
    [13] , , , , , , 2009: Physical simulation experiments on profile control and flooding of crosslinked polymer in edge-water reservoir, Petroleum Geology and Engineering, 23, 109-111+114+8.
    [14] , , , , 2007: Development situation of microbe oil recovery technology, Petroleum Geology and Engineering, 21, 65-68+13-14.
    [15] , , , , , , 2007: Analysis of the influence of oil emulsion drilling fluid on geochemical logging, Petroleum Geology and Engineering, 21, 30-32+8.
    [16] , , 2006: , Petroleum Geology and Engineering, 20, 75-77.
    [17] , , , , , 2005: Potential of indigenous microbial enhanced oil recovery in N3 block of Gangxi oilfield, Petroleum Geology and Engineering, 19, 33-35+99.
    [18] Lu Jun, Zhang Jiqian, 1997: Practice and Understanding of Microbial Enhanced Oil Recovery in Henan Oil Field, Petroleum Geology and Engineering, 11, 23-25+59-60.
    [19] , 1995: , Petroleum Geology and Engineering, 9, 43-43.
    [20] , 1995: , Petroleum Geology and Engineering, 9, 41-41.
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    通讯作者: 陈斌,
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      沈阳化工大学材料科学与工程学院 沈阳 110142

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