Abstract: It is difficult to adjust layering measure and tap the latent potentialities of multi-layer artificial lifted producers,because zonal testing data of surveillance is of ten difficult. In the paper,a new method of using layering water injection of injector to calculate layering production of the relative oil wells is set up to solve the problems, according to principle of injection-production equlibrium integrated with the condition of actual geology and production field data. This method provides a theoretical basis for adjust layering measur andhas a good results with the validation of actual data.