Abstract: The production of natural energy development of tight oil volume fracturing by horizontal wells decreases rapidly, formation energy is insufficient and the recovery factor is low. Therefore, laboratory experiment and field experiment were carried out. The results show that CO_2 huff and puff has good adaptability in tight reservoir development, and it is an effective measure to improve the recovery rate of tight reservoir. The design of injection production parameters of CO_2 huff and puff directly affects the final huff and puff effect. Taking A horizontal well as the laboratory experimental data, this paper optimizes the huff and puff parameters of CO_2 huff and puff timing, total injection volume and injection rate on the basis of production dynamic analysis, through relevant empirical formulas and numerical simulation calculation, and on the basis of evaluation of oil increment and oil exchange rate, so as to provide basis for actual production.