Abstract: Reservoir thickness calculation is an important part in reservoir description but big error can be easily occurred by using common amplitude method. As the reservoir of Daluhu oilfield developed in different times, the reservoir thickness is difficult to be accurately predicted. Under sequence control, based on thin layer reflection tuning principle, tuning frequency and tuning energy body have been formed, which are used for describing reservoir thickness and lateral change. After correlation analysis of real drilling sand body thickness and tuning frequency, the quantitative estimation of reservoir thickness, and the reservoir prediction have been carried out. As a result, the prediction results can provide effective guidance for rolling exploration and achieve good effect of exploration and development.