Abstract: Shale gas is a state of adsorption,free,or dissolve occurrence in mud shale.It belongs to a kind of unconventional natural gas resource.The sedimentary environment gets deeper from the north west to south east in Niutitang Formation of lower Cambrian in southern Guizhou.Profitable sedimentary phase of shale gas is for deep sea shelf-deep sea basin.By means of profile and well testing,the kerogen type of Niutitang Formation has the feature of typeⅠ.Higher organic carbon content,higher thermal evolution degree,lower permeability and developed micro-fracture have good material basis for shale gas reservoirs.Through analysis of evaluation parameters,three areas were optimized for shale gas exploration.