油气微生物数量变化特征模拟实验

杨帆; 许科伟; 顾磊; 宁丽荣; 卢丽; 沈忠民

油气微生物数量变化特征模拟实验

杨帆^1,2,
许科伟^1,2,
顾磊^1,2,
宁丽荣^1,2,
卢丽^1,2,
沈忠民³

1. 中国石化石油勘探开发研究院无锡石油地质研究所, 江苏无锡 214151;
2. 中国石化油气成藏重点实验室, 江苏无锡 214151;
3. 成都理工大学油气藏地质及开发工程国家重点实验室, 四川成都 610059

基金项目:

国家自然科学基金“典型油气藏上方气态烃氧化微生物类群分布异常的深度解析”（41202241）。

详细信息

作者简介:
杨帆(1983-),男,高级工程师,现从事油气地球化学及微生物勘探研究。E-mail:yangfan_56@163.com。

中图分类号: TE357.9
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- 被引次数: 0
出版历程
- 收稿日期: 2019-10-09
- 修回日期: 2020-04-27
- 刊出日期: 2020-12-08

Simulated experimental research on quantity change characteristics of oil and gas microorganisms

YANG Fan^1,2,
XU Kewei^1,2,
GU Lei^1,2,
NING Lirong^1,2,
LU Li^1,2,
SHEN Zhongmin³

1. Wuxi Petroleum Research Institute of Petroleum Geology, SINOPEC, Wuxi, Jiangsu 214151, China;
2. SINOPEC Key Laboratory of Petroleum Accumulation Mechanisms, Wuxi, Jiangsu 214151, China;
3. State Key Lab of Oil-Gas Reservoirs Geology and Exploitation, Chengdu University of Technology, Chengdu, Sichuan 610059, China

摘要

摘要: 以江苏油田某油气区地表土壤样品为介质，构建了确定性油气背景信息的气藏和油藏模拟环境，通过模拟实验研究油气微生物在不同轻烃渗漏条件下的数量变化特征。结果表明，以轻烃为碳源的微生物在数量上呈现较快增长并最终达到平台期，无烃供给条件下，微生物数量逐渐减少。油气微生物数量与轻烃渗漏通量短期内正比，长期驯化后，不同轻烃体积分数所对应的微生物数量趋于一致。气指示基因pmoA和油指示基因bmoX分别对气藏环境和油藏环境具有良好的响应，特异性较强。在无烃供给的枯竭油藏模拟条件下，微生物呈消亡状态。模拟实验印证了地表油气微生物对下伏油气藏具有一定的响应，初步阐明了轻烃渗漏环境下油气微生物数量变化的特征。
- 微生物勘探 /
- 微渗漏 /
- 甲烷氧化菌 /
- 丁烷氧化菌 /
- 模拟实验
Abstract: Based on the surface soil samples of an oil and gas area in Jiangsu oilfield, a gas reservoir and reservoir simulation environment with certain oil and gas background information was constructed. The quantity change characteristics of oil and gas microorganisms under different light hydrocarbon seepage conditions were studied through simulation experiments by using surface soil samples from a certain area in Jiangsu oilfield. The results showed that the number of microorganisms using hydrocarbons as carbon source increased rapidly and finally reached a plateau stage, while the number of microorganisms without hydrocarbon supply decreased gradually. The number of microorganisms was proportional to light hydrocarbon leakage flux in a short time, however, after long-term domestication under different leakage flux, the number of microorganisms tended to be uniform. The abundance of gas indicator gene pmoA and oil indicator gene bmoX have good response and specificity to gas and oil reservoir environment respectively. Microorganisms were in a state of extinction under the simulation condition of exhausted reservoir without hydrocarbon supply. Through simulation experiments, the response of surface oil and gas microorganisms to underlying reservoirs is verified, and the quantitative variation characteristics of oil and gas microorganisms under light hydrocarbon seepage environment are preliminarily clarified．
- microbial exploration /
- microleakage /
- methane oxidizing bacteria /
- butane oxidizing bacteria /
- simulation experiment

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参考文献(23)

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