Abstract: Microbial enhanced oil recovery is a new technique with advantages of small investment, short period, quick effectiveness and fund repay. The recovery mechanism and characteis, the making of well selection criteria and project design, the preparation and implementation of field test.and also the effectiveness analysis for the microbial enhanced oil recovery are presented here. 17 wells were tested by huff- puff and backflow methods. Accumulative incremental oil production was up to 1455 tons and water cut was reduced to a certain degree after injection of 5940 liters of microbial solution. As a result, the fluid property of crude oil has improved and the economic benefit was notable, that has laid down a foundation for large scale ap plication.