Abstract: There are many problems and disadvantages in the old procession of water separate injection in horizontal wells. The problems mentioned hereof including but not limited in the follows. It is hard to open the packer & fishing, short period of validity, and unable to do dropping-fishing test. Therefore, the procession by injecting the water to the well section by section is introduced to reduce the force of unsealing and improve the success rate of unsealing. By adopting backwashing and balancing mechanism, to reduce the wriggle of the string and prolong its validity period. Wireless testing and adjusting technology were used to realize the layered testing of underground flow and pressure. The flow rate and pressure of the water was well controlled while working. The property of the isolation packer showed as the follows: the max pressure of 25 MPa, the max temperature of 120℃, and the max loading while open the packer of-50 kN. With this wireless testing and adjusting technique, the down hole flow of water injection in 5 sections was monitored. The flow accuracy error is controlled within 5%, the pressure accuracy error is within 0.3%, and the validity period is up to 2 years. The field application shows that the multi-segment separate water injection technology has good separate injection effect, simple measurement and adjustment, and improves the degree of fine water injection.